operetta high content imaging system (Revvity)
Revvity is a verified supplier 
Revvity manufactures this product 
96
Structured Review
Revvity
operetta high content imaging system
Operetta High Content Imaging System, supplied by Revvity, used in various techniques. Bioz Stars score: 96/100, based on 3428 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/image+analysis+system/Operetta+CLS+high-content+analysis+system/pmc13657315-62-1-5
Average 96 stars, based on 3428 article reviews
Operetta High Content Imaging System, supplied by Revvity, used in various techniques. Bioz Stars score: 96/100, based on 3428 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/image+analysis+system/Operetta+CLS+high-content+analysis+system/pmc13657315-62-1-5
Average 96 stars, based on 3428 article reviews
operetta high content imaging system - by Bioz Stars,
2026-10
96/100 stars
Images
1) Product Images from "Omicron SARS-CoV-2 shows NRP1-linked susceptibility in adipose-derived MSCs and alters primitive cord blood CD34⁺ HSPCs"
Article Title: Omicron SARS-CoV-2 shows NRP1-linked susceptibility in adipose-derived MSCs and alters primitive cord blood CD34⁺ HSPCs
Journal: PLOS One
doi: 10.1371/journal.pone.0360439
Figure Legend Snippet: (A) Detection of SARS-CoV-2 infected cells using high-content imaging under control conditions. Fluorescent signals: green (anti-SARS-CoV NP mAb), blue (Hoechst). (B) Representative flow cytometry analysis plots showing NP+ cells. (C) Measurement of fluorescent intensity in NP+ cells. (D) Percentage of infected MSCs. (E) The supernatants of infected MSCs were collected and titrated on Vero-E6/TMPRSS2 cells to measure infectious viral particle production using the focus-forming assay. (F) After RNA extraction from infected MSCs, the expression of IDO1 was examined using real-time PCR. The graph displays the expression of IDO1 normalized to GAPDH , with the mock group serving as the control. Data are represented as means ± SEM from three independent biological replicates. For , the Delta condition was available from only two independent donors and was therefore presented descriptively and excluded from the statistical analysis. Statistical comparisons among the remaining groups were performed using one-way ANOVA followed by Tukey’s multiple-comparisons test. One-way ANOVA showed a significant overall difference among groups, F(2, 6) = 6.794, P = 0.0287. * P < 0.05, ** P < 0.01, *** P = 0.0001, **** P < 0.0001.
Techniques Used: Infection, Imaging, Control, Flow Cytometry, Analysis, Focus Forming Assay, RNA Extraction, Expressing, Real-time Polymerase Chain Reaction
Figure Legend Snippet: (A) Representative western blot depicting the expression of NRP1 in AD-MSCs (No siRNA), cells transfected with non-targeting siRNA (siControl), or siNRP1 for 24 hours. β-actin was used as a loading control. (B) Graph displaying the quantification of NRP1 normalized to β-actin (n = 3). This experiment represents three independent biological replications. Statistical analysis was performed using one-way ANOVA followed by Tukey’s multiple comparisons test. (C) Transfected cells were visualized using high-content imaging. Fluorescent signals: green for anti-NRP1 mAb, blue for Hoechst. (D) Percentage of NRP1-expressing cells normalized to cells transfected with siControl. Data are presented as mean ± SEM from three independent experiments. Statistical analysis was conducted using a paired t-test. Next, siRNA-transfected cells were infected with different variants of SARS-CoV-2. (E) SARS-CoV-2-infected cells were detected using high-content imaging. Fluorescent signals: green for anti-SARS-CoV NP mAb, blue for Hoechst. (F) The fluorescent intensity of NP+ cells was measured. All data represent three independent experiments. Statistical analysis among each group of variant was performed using an unpaired t-test. * P < 0.05, ** P < 0.01, *** P = 0.0001, **** P < 0.0001.
Techniques Used: Western Blot, Expressing, Transfection, Control, Analysis, Imaging, Infection, Variant Assay
Related Articles
Infection:Article Title: Concanavalin A targets phylogenetically conserved N-linked glycans on coronavirus spike proteins for broad-spectrum antiviral activity. Article Snippet: Images of the cells were captured at endpoint using a 10x objective and a 12-bit monochrome complementary metal oxide semiconductor (CMOS) camera installed on the CKX53 inverted microscope (Olympus). .. The area of Caco-2 cell infection was quantified as image region areas using the Imaging:Article Title: Concanavalin A targets phylogenetically conserved N-linked glycans on coronavirus spike proteins for broad-spectrum antiviral activity. Article Snippet: Images of the cells were captured at endpoint using a 10x objective and a 12-bit monochrome complementary metal oxide semiconductor (CMOS) camera installed on the CKX53 inverted microscope (Olympus). .. The area of Caco-2 cell infection was quantified as image region areas using the Article Title: Beauvericin promotes autophagy and mitophagy by activating NIPSNAP2. Article Snippet: Dysregulation of autophagy and mitophagy is a hallmark of neurodegenerative diseases, including Alzheimer's disease (AD).. Chemical intervention targeting these pathways has emerged as one of the promising therapeutic strategies for neurodegenerative disorders.. Here, we identified beauvericin as a candidate molecule that regulates autophagy and mitophagy through an organelle phenotypes-based high-throughput screening of a marine natural products library. High Content Screening:Article Title: Tannic acid inhibits viral replication by disrupting nucleocapsid condensation Article Snippet: Background: Viruses continue to threaten global health, highlighting the urgent need for antiviral strategies with broad-spectrum activity.. The nucleocapsid (N) protein undergoes liquid–liquid phase separation (LLPS), a conserved process essential for viral assembly.. However, pharmacological targeting of this process remains largely unexplored. Staining:Article Title: Beauvericin promotes autophagy and mitophagy by activating NIPSNAP2. Article Snippet: Dysregulation of autophagy and mitophagy is a hallmark of neurodegenerative diseases, including Alzheimer's disease (AD).. Chemical intervention targeting these pathways has emerged as one of the promising therapeutic strategies for neurodegenerative disorders.. Here, we identified beauvericin as a candidate molecule that regulates autophagy and mitophagy through an organelle phenotypes-based high-throughput screening of a marine natural products library. Analysis:Article Title: Beauvericin promotes autophagy and mitophagy by activating NIPSNAP2. Article Snippet: Dysregulation of autophagy and mitophagy is a hallmark of neurodegenerative diseases, including Alzheimer's disease (AD).. Chemical intervention targeting these pathways has emerged as one of the promising therapeutic strategies for neurodegenerative disorders.. Here, we identified beauvericin as a candidate molecule that regulates autophagy and mitophagy through an organelle phenotypes-based high-throughput screening of a marine natural products library. Liquid Chromatography:Article Title: A sweet cherry zinc finger transcription factor, PaZFP20, positively regulates salt tolerance in transgenic poplar via modulating hormone metabolism. Article Snippet: Soil salinity severely impacts plant growth and productivity.. C3H-type zinc finger transcription factors (TFs) are known to play crucial roles in abiotic stress responses, but their functional characterization in fruit trees like sweet cherry remains limited.. Here, we identified the C3H-type zinc finger protein (ZFP) family in the sweet cherry (Prunus avium) genome and characterized a salt-responsive member, PaZFP20 (Prunus avium zinc finger protein 20). Targeted Proteomics:Article Title: A sweet cherry zinc finger transcription factor, PaZFP20, positively regulates salt tolerance in transgenic poplar via modulating hormone metabolism. Article Snippet: Soil salinity severely impacts plant growth and productivity.. C3H-type zinc finger transcription factors (TFs) are known to play crucial roles in abiotic stress responses, but their functional characterization in fruit trees like sweet cherry remains limited.. Here, we identified the C3H-type zinc finger protein (ZFP) family in the sweet cherry (Prunus avium) genome and characterized a salt-responsive member, PaZFP20 (Prunus avium zinc finger protein 20). Mass Spectrometry:Article Title: A sweet cherry zinc finger transcription factor, PaZFP20, positively regulates salt tolerance in transgenic poplar via modulating hormone metabolism. Article Snippet: Soil salinity severely impacts plant growth and productivity.. C3H-type zinc finger transcription factors (TFs) are known to play crucial roles in abiotic stress responses, but their functional characterization in fruit trees like sweet cherry remains limited.. Here, we identified the C3H-type zinc finger protein (ZFP) family in the sweet cherry (Prunus avium) genome and characterized a salt-responsive member, PaZFP20 (Prunus avium zinc finger protein 20). |